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當前位置:上海富雨生物科技有限公司>>細胞庫 / 細胞培養>>細胞株>> A549/Taxol人肺癌紫杉
細胞系特征 | ||
細胞株名稱:A549/Taxol人肺癌耐藥株 種屬:人 組織來源:肺癌 生長特性:貼壁生長 形態特征:上皮細胞 微生物及支原體檢測:陰性 安全性:所有腫瘤和病毒轉染的細胞均視為有潛在的生物危害性,必須在二級生物安全臺內操作,并請注意防護,所有廢液及接觸過此細胞的器皿需高壓滅菌后方能丟棄。 | ||
培養條件:
| 培養基:90%RPMI-1640+10%胎牛血清+100ng/ml Taxol 血清我們推薦用: GIBCOFBS-10099-141或HYCLONEFBS-SH30084.03。 培養條件:37.0C carbon dioxide(CO2),5% | |
傳代方法:
| 收到細胞后,在倒置鏡下(是在4X物鏡)觀察整個細胞生長情況。 (一)如果細胞未長滿,用75%酒精噴灑整個瓶消毒后放到超菌臺內,嚴格無菌操作,打開細胞培養瓶,吸出培養液,換 10ml新鮮培養液后繼續培養。 (二)如果細胞已長滿,即可進行傳代培養。具體步驟如下: 1. 棄去培養液,用PBS(不含鈣,鎂離子)洗1-2次。 2. 加0.7-1ml消化液(0.25%Trypsin-0.53mM EDTA)于培養瓶中,用力拍打瓶壁,期間每隔 5-10s放到顯微鏡下觀察,直至50-70%的細胞脫落后,加入2ml 以上培養基中止消化。 3. 按6-8ml/瓶補加培養基,輕輕打勻后吸出一半,分到新的培養瓶中。如果沒有特別說明,收到細胞后的次傳代一般是一傳二。 注:1、觀察細胞在低倍鏡(4或5X物鏡)下進行,否則不能準確判斷細胞的傳代密度。看細胞的形態請在10X或20X物鏡下。 2、瓶中運輸培養基不能重復再用,請換用加雙抗的新培養基,細胞凍存后,培養基中可不加任何抗生素。 3、有些細胞貼壁不牢,如發現貼壁細胞有脫落,可離心吹打后接種到新瓶內。 4、收到細胞后,若發現培養瓶破損、有液溢出及細胞有污染,請及時與我們聯系。..
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凍存方法: | 凍存液:90%胎牛血清,10%DMSO 儲存:液氮儲存 |
LRG1,HIF-1 α and Nrf-2 on the first and third days after operation in the infection group were higher than
those before operation,the level of serup serup LRG1,HIF-1 α and Nrf-2 on the first day after operation
in the infected group were higher than that on the third day after operation,the digerences were statistically
significant ( P <0.05) ; the level of serup Nrf-2 on the first day after operation in the
Although the activity of autophagy is maintained at lower levels in resting T cells, it is
markedly upregulated upon activation [3–6]. The potential purposes of this upregulation are summarized in Figure 2. Using a variety of autophagy-deficient T cell models, several
groups have demonstrated a key homeostatic role of autophagy in T cell proliferation and survival [6, 78–81]. Based on ocdfrvations that T-cell-specific Atg3-, Atg5-, and Atg7-
deficient mice had reduced numbers of CD4+ and CD8+ T cells in the spleen and lymph
nodes, it was also determined that autophagy-deficient T cells showed increased rates of
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